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Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings BJM
Dayanandan,A.; Rani,S. Hilda Vimala; Shanmugavel,M.; Gnanamani,A.; Rajakumar,G. Suseela.
The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) gives an increased enzyme production of 793 ± 6.56 u/g. The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C. Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat bran; Oil cakes; Lipase; Tannery fleshing; Fat hydrolysis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400010
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